In general, pipettes require regular inspection. Regular inspection is mainly to ensure the better accuracy of the pipette and the inspection results. The pipette is a measure-out type, which is greatly affected by the environment and operation. Therefore, when adjusting the range of the pipette, the counterclockwise rotation is used in order to ensure the accuracy of the measurement during the rotation of the knob. Use your index finger and thumb to adjust the knob. If the maximum range is exceeded, adjustment of the knob is also required. In addition, special attention should be paid to the adjustment accuracy taking into account the damage to the pipette caused by the internal mechanical jamming. Therefore, when assembling the liquid gun, pay attention to the assembly process of the pipette tip to avoid loosening of internal parts. When installing the pipette tip, pay attention to the installation method of the pipette tip. It needs to be inserted vertically and rotated slightly left and right at the same time to help the knob match with the pipette tip. In addition, two aspects should be considered for the unqualified sealing. The first is the pipette tip. Consider checking the installation of the tip. Pay attention to whether the tip matches with the instrument or there is an installation error, or if there is a problem in the system itself. Replacing the tip can help achieve the purpose of the smooth progress of the inspection. The second is to check that the gap between the internal sealing ring of the pipette and the piston is too big, which may be related to the aging of the sealing ring. The leakage caused by this problem can be solved by applying a small amount of Vaseline evenly on the piston, or using the same sealing ring to help solve the problem of sealing. If there is still a leakage after applying Vaseline on the piston, it is necessary to replace the sealing ring of the same type.
In addition, when solving the problem of volume error, the volume of the pipette can be adjusted; the occurrence of volume error can be identified, and the volume can be increased or decreased by the Allen wrench equipped with the pipette itself. First, the current capacity value needs to be verified, and the pipette needs to be adjusted to the minimum verification point. The difference between the current capacity value and the marked capacity value needs to be calculated, and the adjustment hole can be adjusted with a wrench. Detection should be performed after adjustment. Through calculation and multiple measurements, observe whether it conforms to the verification regulations. If the corresponding requirements are met, continue to inspect. The pipette can be adjusted to the middle inspection point. If the middle inspection point meets the verification requirements, the maximum verification point can be detected, and the detection and adjustment can be carried out several times to determine whether the different verification points are correct. In accordance with the requirements of the regulations, multiple corrections and tests can be carried out until the values of the three verification points meet the corresponding verification regulations.
Conclusion
A pipette is a highly accurate measuring instrument. Its accuracy will directly affect the experimental results. Therefore, it is necessary to carry out corresponding verification of the pipette, and to correct the technical problems in time to ensure that the measured data is more accurate and reliable. At the same time, it is also necessary to pay attention to the use of the instrument and operate strictly to prolong the service life of the pipette.
In addition, when solving the problem of volume error, the volume of the pipette can be adjusted; the occurrence of volume error can be identified, and the volume can be increased or decreased by the Allen wrench equipped with the pipette itself. First, the current capacity value needs to be verified, and the pipette needs to be adjusted to the minimum verification point. The difference between the current capacity value and the marked capacity value needs to be calculated, and the adjustment hole can be adjusted with a wrench. Detection should be performed after adjustment. Through calculation and multiple measurements, observe whether it conforms to the verification regulations. If the corresponding requirements are met, continue to inspect. The pipette can be adjusted to the middle inspection point. If the middle inspection point meets the verification requirements, the maximum verification point can be detected, and the detection and adjustment can be carried out several times to determine whether the different verification points are correct. In accordance with the requirements of the regulations, multiple corrections and tests can be carried out until the values of the three verification points meet the corresponding verification regulations.
Conclusion
A pipette is a highly accurate measuring instrument. Its accuracy will directly affect the experimental results. Therefore, it is necessary to carry out corresponding verification of the pipette, and to correct the technical problems in time to ensure that the measured data is more accurate and reliable. At the same time, it is also necessary to pay attention to the use of the instrument and operate strictly to prolong the service life of the pipette.
next: Chemical Anti-fogging of Goggles for Coronavirus
previous: Research on Common Technical Problems and Solutions in Pipettes
