In the laboratory, the pipette is an important tool, which directly affects the accuracy and consistency of experimental results. However, due to various reasons, there are often errors in the pipetting process, which may seriously affect the quality of experimental data. Understanding and avoiding these common pipetting errors is key to ensuring the reliability of laboratory operations. Here are five common pipetting mistakes and how to prevent them. Use an Uncalibrated Pipette Over time, the internal...

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qPCR technology is a key tool in gene expression analysis, mutation detection and pathogen detection. This paper will introduce in detail the detection of cell inflammatory factors by qRT-PCR, the required materials and equipment, experimental procedures and precautions to help researchers better grasp and apply this key technology. Principles of qPCR Quantitative polymerase chain reaction (qPCR) is a molecular biological technique used to quantitatively analyze the target DNA sequence in a...

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A centrifuge tube is a tubular container for laboratory samples commonly used in experimental processes such as centrifugation, sample preservation, and liquid replacement. Centrifugal tubes are available in various sizes and can be selected according to specific experimental requirements. These small test tubes play an important role in scientific research and medical experiments, and their main function is to separate different substances based on density. In recent years, significant advances...

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PCR (Polymerase chain reaction) is a very sensitive molecular biology technique capable of producing a large number of copies of DNA from a tiny amount of starting material. This makes PCR a basic and efficient experimental method. However, due to its susceptibility to amplicon and sample contamination, special careful consideration is required when planning and designing PCR laboratories. Laboratory Layout An ideal PCR lab would consist of two rooms and two areas, each designed for a specific task....

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DNA is the basic blueprint for the health and genetic information of living organisms. The ability to decode these genetic signatures has transformed scientific knowledge and medical practice. At the heart of this breakthrough is polymerase chain reaction (PCR), a powerful technique that we will explore in this article. Before the advent of PCR, using DNA was often difficult, inefficient, and expensive. Researchers often face the challenge of small sample sizes, and replicating DNA involves a lengthy...

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The centrifuge tube used in the laboratory is a key tool for sample separation and purification. Choosing the right centrifuge tube is crucial to ensure the accuracy, efficiency and safety of the experiment. The following are a series of detailed factors to consider when choosing a centrifuge tube, which will also be standard in the product description page, read these marks to help you choose a more suitable centrifuge tube. Different Elements of Centrifuge Tubes Capacity and Size Centrifugal tubes...

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In biological laboratories and pharmaceutical R&D, workstation pipette tips are commonly used as experimental tools, and their proper selection and use are crucial to the accuracy and reliability of experimental results. In this article, we will discuss in detail the selection criteria and precautions for the use of workstation pipette tips to help laboratory personnel better master this experimental technique. Selection of Pipette Tips  First, when choosing a workstation pipette tip,...

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As an important experimental tool, gel-loaded pipette tip plays an irreplaceable role in various fields such as biochemistry, molecular biology, genetics, etc. It is highly favored by researchers because of its unique design features and excellent experimental performance. This article will introduce the characteristics and experimental applications of gel-loaded pipette tips in detail.  Characteristics of Gel-Loaded Pipette Tips  The design of the gel loading pipette tip is...

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PCR plates remain a cornerstone in the lab, with their significance amplified by the rise of high-throughput and automated workflows. Yet, the drive for efficiency can complicate the preservation of experimental precision, particularly in the critical step of sealing PCR plates. Inadequate sealing can result in sample loss through evaporation, shifts in pH that can impair enzymatic reactions, and the risk of contamination. Thus, perfecting the technique for sealing PCR plates is essential for maintaining...

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